产品详情
Isotype（亚型） | IgG |
Calculated MW（分子量） | 理论分子量42kDa/44kDa； 实际分子量42kDa/44kDa |
Immunogen（免疫原） | Recombinant protein (or fragment). |
Gene ID | 5594/5595 |
Swiss Prot | P28482/P27361 |
Purification Method（纯化方式） | Affinity purification |
Subcellular location（亚细胞定位） | caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane. |
Applications（应用）
Species reactivity（反应种属）
Human Mouse Rat
Tested Applications（已测试应用）
WB IHC-P IF/ICC IF-F ELISA
Recommended Dilutions（推荐稀释比例）
Tested Applications | Recommended Dilutions |
WB | 1:1000-1:3000 |
IHC-P | 1:2000-1:8000 |
IF/ICC | 1:100-1:200 |
IF-F | 1:50-1:500 |
ELISA | Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
Storage（储存）
Storage Buffer（储存缓冲液）
PBS with 0.09% sodium azide,0.05%BSA,50% glycerol,pH7.3.
Storage Instruction（储存条件）
Shiped at 4℃.Short term 4℃(1-2weeks).It isrecommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Background Information（背景信息）
This gene encodes a MAP kinase family member, also known as extracellular signal-regulated kinase (ERK). ERKs integrate diverse biochemical signals and regulate multiple cellular processes such as proliferation, differentiation, transcription and development. This kinase is activated via phosphorylation by upstream kinases, then translocates to the nucleus to phosphorylate nuclear substrates upon activation. It can also act as a transcriptional repressor independent of its kinase activity, classifying it as a moonlighting protein. Two alternatively spliced transcript variants have been identified; they produce the same protein but differ in untranslated regions.
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