产品详情
Isotype（亚型） | IgG |
Calculated MW（分子量） | 理论分子量13kDa；实际分子量15-20kDa |
Immunogen（免疫原） | Recombinant protein (or fragment). |
Gene ID | 1978 |
Swiss Prot | Q13541 |
Purification Method（纯化方式） | Affinity purification |
Subcellular location（亚细胞定位） | Cytoplasm, Nucleus |
Applications（应用）
Species reactivity（反应种属）
Human Mouse Rat
Tested Applications（已测试应用）
WB IHC-P ELISA
Recommended Dilutions（推荐稀释比例）
Tested Applications | Recommended Dilutions |
WB | 1:1500-1:10000 |
IHC-P | 1:100-1:1000 |
ELISA | Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
Storage（储存）
Storage Buffer（储存缓冲液）
PBS with 0.09% sodium azide,0.05%BSA,50% glycerol,pH7.3.
Storage Instruction（储存条件）
Shiped at 4℃.Short term 4℃(1-2weeks).It isrecommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Background Information（背景信息）
4EBP1 (4E-BP1) is a translational regulator that binds and sequesters eIF4E to block translation initiation. It harbors multiple phosphorylation sites including Thr37, Thr46, Ser65, Thr70, Ser83 and Ser112; Thr37, Thr46, Ser65 and Thr70 are modulated by mTOR signaling. Phosphorylation at Thr37 and Thr46 primes 4EBP1 for subsequent phosphorylation at Ser65 and Thr70, leading to its dissociation from eIF4E.
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