产品详情
Isotype（亚型） | IgG |
Calculated MW（分子量） | 理论分子量56kDa；实际分子量60kDa |
Immunogen（免疫原） | Recombinant protein (or fragment). |
Gene ID | 207 |
Swiss Prot | P31749 |
Purification Method（纯化方式） | Affinity purification |
Subcellular location（亚细胞定位） | Cell membrane, Cytoplasm, Nucleus. |
Applications（应用）
Species reactivity（反应种属）
Human Mouse Rat
Tested Applications（已测试应用）
WB IHC-P ELISA
Recommended Dilutions（推荐稀释比例）
Tested Applications | Recommended Dilutions |
WB | 1:1000-1:3000 |
IHC-P | 1:50 - 1:400 |
ELISA | Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
Storage（储存）
Storage Buffer（储存缓冲液）
PBS with 0.09% sodium azide,0.05%BSA,50% glycerol,pH7.3.
Storage Instruction（储存条件）
Shiped at 4℃.Short term 4℃(1-2weeks).It isrecommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Background Information（背景信息）
This gene encodes AKT1, one of three human AKT serine-threonine kinase isoforms (PKB α/β/γ). All AKT members share an N-terminal pleckstrin homology domain, a serine/threonine kinase domain and a C-terminal regulatory domain. Activated downstream of PI3K, AKT is recruited to the plasma membrane by PIP3, where dual phosphorylation at Thr308 and Ser473 is required for full activation. Additional phosphorylation can be triggered by IGF-1 and EGF, while phosphatases negatively regulate this pathway via dephosphorylation of AKT or PIP3. The PI3K/AKT axis governs cell proliferation, survival, metabolism and angiogenesis; it also acts upstream of mTOR to control cap-dependent translation. AKT1 suppresses apoptosis by phosphorylating and inhibiting apoptotic effectors. Dysregulation or mutation of AKT1 occurs in multiple malignancies as well as overgrowth disorders including Proteus syndrome and Cowden syndrome 6. Multiple alternatively spliced transcripts of AKT1 have been identified.
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