产品详情
Isotype（亚型） | IgG |
Calculated MW（分子量） | 理论分子量56kDa；实际分子量60kDa |
Immunogen（免疫原） | Recombinant protein (or fragment). |
Gene ID | 207/208/10000 |
Swiss Prot | P31749/P31751/Q9Y243 |
Purification Method（纯化方式） | Affinity purification |
Subcellular location（亚细胞定位） | ciliary basal body, cytoplasm, cytosol, microtubule cytoskeleton, mitochondrion, nucleoplasm, nucleus, plasma membrane, spindle. |
Applications（应用）
Species reactivity（反应种属）
Human Mouse Rat
Tested Applications（已测试应用）
WB IHC-P IF/ICC IF-P ELISA
Recommended Dilutions（推荐稀释比例）
Tested Applications | Recommended Dilutions |
WB | 1:1000-1:6000 |
IHC-P | 1:100-1:400 |
IF/ICC | 1:50-1:200 |
IF-P | 1:50-1:200 |
ELISA | Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
Storage（储存）
Storage Buffer（储存缓冲液）
PBS with 0.09% sodium azide,0.05%BSA,50% glycerol,pH7.3.
Storage Instruction（储存条件）
Shiped at 4℃.Short term 4℃(1-2weeks).It isrecommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Background Information（背景信息）
This gene encodes AKT1, one of three human AKT serine-threonine kinase isoforms (PKB α, β, γ). AKT members share an N-terminal PH domain, a kinase domain and a C-terminal regulatory domain. Downstream of PI3K, AKT is recruited to the membrane by PIP3 converted from PIP2. Full activation requires phosphorylation at Thr308 and Ser473; IGF-1 and EGF promote phosphorylation, while phosphatases suppress the pathway. The PI3K/AKT axis responds to extracellular ligands and regulates cell proliferation, survival, metabolism and angiogenesis. Activated AKT inhibits apoptosis transcription-independently and modulates the eIF4E complex upstream of mTOR. AKT mutations and pathway dysregulation are associated with cancers and overgrowth syndromes. Multiple splice variants are present.
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