产品详情
Isotype（亚型） | IgG |
Calculated MW（分子量） | 理论分子量48kDa/55kDa/51kDa/54kDa； 实际分子量60kDa |
Immunogen（免疫原） | Recombinant protein (or fragment). |
Gene ID | 207/208/10000 |
Swiss Prot | P31749/P31751/Q9Y243 |
Purification Method（纯化方式） | Affinity purification |
Subcellular location（亚细胞定位） | Ciliary basal body, Cytoplasm, Cytosol, Microtubule cytoskeleton, Mitochondrion, Nucleoplasm, Nucleus, Plasma membrane, Spindle. |
Applications（应用）
Species reactivity（反应种属）
Human Mouse Rat
Tested Applications（已测试应用）
WB ELISA
Recommended Dilutions（推荐稀释比例）
Tested Applications | Recommended Dilutions |
WB | 1:1000-1:2500 |
ELISA | Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
Storage（储存）
Storage Buffer（储存缓冲液）
PBS with 0.09% sodium azide,0.05%BSA,50% glycerol,pH7.3.
Storage Instruction（储存条件）
Shiped at 4℃.Short term 4℃(1-2weeks).It isrecommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Background Information（背景信息）
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
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