产品详情
Isotype（亚型） | IgG |
Calculated MW（分子量） | 理论分子量48kDa/55kDa/51kDa/54kDa； 实际分子量60kDa |
Immunogen（免疫原） | Recombinant protein (or fragment). |
Gene ID | 207/208/10000 |
Swiss Prot | P31749/P31751/Q9Y243 |
Purification Method（纯化方式） | Affinity purification |
Subcellular location（亚细胞定位） | ciliary basal body, cytoplasm, cytosol, microtubule cytoskeleton, mitochondrion, nucleoplasm, nucleus, plasma membrane, spindle. |
Applications（应用）
Species reactivity（反应种属）
Human Mouse Rat
Tested Applications（已测试应用）
WB IHC-P ELISA
Recommended Dilutions（推荐稀释比例）
Tested Applications | Recommended Dilutions |
WB | 1:1000-1:2000 |
IHC-P | 1:50-1:100 |
ELISA | Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.For high-ratio antibody dilutions (≥1:10000),a sequential dilution method is strongly recommended to ensure measurement accuracy. |
Storage（储存）
Storage Buffer（储存缓冲液）
PBS with 0.09% sodium azide,0.05%BSA,50% glycerol,pH7.3.
Storage Instruction（储存条件）
Shiped at 4℃.Short term 4℃(1-2weeks).It isrecommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Background Information（背景信息）
The AKT1 gene encodes a serine-threonine kinase that remains catalytically inactive in serum-deprived primary and immortalized fibroblasts. AKT1 and its homolog AKT2 are rapidly and specifically activated by platelet-derived growth factor, a process dependent on phosphatidylinositol 3 kinase and abolished by mutations within the pleckstrin homology domain of AKT1. In the developing nervous system, AKT serves as a key mediator of growth factor-supported neuronal survival. Survival factors inhibit apoptosis in a transcription-independent way by activating AKT1; activated AKT1 phosphorylates and deactivates apoptotic effectors. Variants of this gene are linked to Proteus syndrome. Several alternatively spliced transcript variants have been identified.
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