产品详情
Isotype（亚型） | IgG |
Calculated MW（分子量） | 理论分子量42-54kDa/81-84kDa； 实际分子量85kDa |
Immunogen（免疫原） | Recombinant protein (or fragment). |
Gene ID | 5295/5296/8503 |
Swiss Prot | P27986/O00459/Q92569 |
Purification Method（纯化方式） | Affinity purification |
Subcellular location（亚细胞定位） | Golgi network, Cytoplasm, Cytosol, Nucleus, Perinuclear region of cytoplasm, Plasma membrane. |
Applications（应用）
Species reactivity（反应种属）
Mouse Rat
Tested Applications（已测试应用）
WB IF/ICC ELISA
Recommended Dilutions（推荐稀释比例）
Tested Applications | Recommended Dilutions |
WB | 1:2500 - 1:9000 |
IF/ICC | 1:50 - 1:100 |
ELISA | Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
Storage（储存）
Storage Buffer（储存缓冲液）
PBS with 0.09% sodium azide,0.05%BSA,50% glycerol,pH7.3.
Storage Instruction（储存条件）
Shiped at 4℃.Short term 4℃(1-2weeks).It isrecommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Background Information（背景信息）
Phosphatidylinositol 3 kinase adds phosphate groups to the 3' position of the inositol ring of phosphatidylinositol. This enzyme is composed of a 110 kDa catalytic subunit and a regulatory subunit of 85 kDa, 55 kDa or 50 kDa. This gene encodes the 85 kDa regulatory subunit. PI3K is essential for insulin driven metabolic functions, and mutations in this gene are linked to insulin resistance. Alternative splicing yields four transcript variants encoding separate isoforms.
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