产品详情
Isotype（亚型） | IgG |
Calculated MW（分子量） | 理论分子量60kDa；实际分子量65kDa |
Immunogen（免疫原） | Recombinant protein (or fragment). |
Gene ID | 5970 |
Swiss Prot | Q04206 |
Purification Method（纯化方式） | Affinity purification |
Subcellular location（亚细胞定位） | Cytoplasm, Nucleus. |
Applications（应用）
Species reactivity（反应种属）
Human Mouse Rat Monkey
Tested Applications（已测试应用）
WB IHC-P IF/ICC IF-F ELISA
Recommended Dilutions（推荐稀释比例）
Tested Applications | Recommended Dilutions |
WB | 1:2500 - 1:10000 |
IHC-P | 1:1000 - 1:4000 |
IF/ICC | 1:300 - 1:1200 |
IF-F | 1:50 - 1:200 |
ELISA | Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
Storage（储存）
Storage Buffer（储存缓冲液）
PBS with 0.09% sodium azide,0.05%BSA,50% glycerol,pH7.3.
Storage Instruction（储存条件）
Shiped at 4℃.Short term 4℃(1-2weeks).It isrecommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Background Information（背景信息）
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
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