服务介绍
Isotype（亚型） | IgG |
Calculated MW（分子量） | 理论分子量42kDa/44kDa； 实际分子量42kDa/44kDa |
Immunogen（免疫原） | Recombinant protein (or fragment). |
Gene ID | 5595/5594 |
Swiss Prot | P27361/P28482 |
Purification Method（纯化方式） | Affinity purification |
Subcellular location（亚细胞定位） | caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, focal adhesion, Golgi apparatus, late endosome, mitochondrion, nuclear envelope, nucleoplasm, nucleus, plasma membrane. |
Applications（应用）
Species reactivity（反应种属）
Human Mouse Rat
Tested Applications（已测试应用）
WB IHC-P IF/ICC ELISA
Recommended Dilutions（推荐稀释比例）
Tested Applications | Recommended Dilutions |
WB | 1:1000-1:2500 |
IHC-P | 1:100-1:400 |
IF/ICC | 1:100-1:400 |
ELISA | Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
Storage（储存）
Storage Buffer（储存缓冲液）
PBS with 0.09% sodium azide,0.05%BSA,50% glycerol,pH7.3.
Storage Instruction（储存条件）
Shiped at 4℃.Short term 4℃(1-2weeks).It isrecommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Background Information（背景信息）
This protein is a member of the MAP kinase family, also known as extracellular signal-regulated kinases (ERKs). ERKs participate in signaling cascades to regulate cell proliferation, differentiation and cell cycle progression in response to extracellular stimuli. Activated by upstream phosphorylation, it translocates to the nucleus and phosphorylates nuclear substrates. Alternatively spliced transcripts encoding different isoforms have been identified.
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