服务介绍
Isotype（亚型） | IgG |
Calculated MW（分子量） | 理论分子量113kDa；实际分子量140-150kDa |
Immunogen（免疫原） | Recombinant protein (or fragment). |
Gene ID | 8408 |
Swiss Prot | O75385 |
Purification Method（纯化方式） | Affinity purification |
Subcellular location（亚细胞定位） | Cytoplasm, preautophagosomal structure, cytosol. |
Applications（应用）
Species reactivity（反应种属）
Human Mouse
Tested Applications（已测试应用）
WB IF/ICC ELISA
Recommended Dilutions（推荐稀释比例）
Tested Applications | Recommended Dilutions |
WB | 1:1000-1:4000 |
IF/ICC | 1:50-1:100 |
ELISA | Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
Storage（储存）
Storage Buffer（储存缓冲液）
PBS with 0.09% sodium azide,0.05%BSA,50% glycerol,pH7.3.
Storage Instruction（储存条件）
Shiped at 4℃.Short term 4℃(1-2weeks).It isrecommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Background Information（背景信息）
Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.
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